敖宇飞 副研究员
敖宇飞 副研究员
学习与工作经历
2003 - 2007 北京师范大学 获得学士学位
2008 - 2014 中国科学院化学研究所 获得博士学位（师从王梅祥教授）
2014 - 2019 中国科学院化学研究所 助理研究员
2019 - 至今 中国科学院化学研究所 副研究员
2021.3 - 2023.3 Greifswald大学 访问学者 (Uwe T. Bornscheuer教授课题组)
研究领域
联系方式
中国科学院化学研究所分子识别与功能院重点实验室
地址：北京市海淀区中关村北一街2号5#1015 邮编: 100190
邮箱：aoyufe@iccas.ac.cn
电话：010-62565610
ORCID：https://orcid.org/0000-0002-9415-2181
发表论文
Zi-Lin Li, Shuxin Pei, Ziying Chen, Teng-Yu Huang, Xu-Dong Wang, Lin Shen,* Xuebo Chen,* Qi-Qiang Wang, De-Xian Wang, Yu-Fei Ao,* Machine learning-assisted amidase-catalytic enantioselectivity prediction and rational design of variants for improving enantioselectivity, Nat. Commun. 2024, 15, 8778.
Chenghai Sun,* Gen Lu, Baoming Chen, Guangjun Li, Ya Wu, Yannik Brack, Dong Yi, Yu-Fei Ao, Shuke Wu, Ren Wei, Yuhui Sun, Guifa Zhai,* Uwe T. Bornscheuer,* Direct asymmetric synthesis of β-branched aromatic α-amino acids using engineered phenylalanine ammonia lyases, Nat. Commun. 2024, 15, 8264.
Marian J. Menke, Yu-Fei Ao,* Uwe T. Bornscheuer,* Practical Machine Learning-Assisted Design Protocol for Protein Engineering: Transaminase Engineering for the Conversion of Bulky Substrates, ACS Catal. 2024, 14, 6462-6469.
Yu-Fei Ao,* Mark Dӧrr, Marian J. Menke, Stefan Born, Egon Heuson, Uwe T. Bornscheuer,* Data-Driven Protein Engineering for Improving Catalytic Activity and Selectivity, ChemBioChem 2024, 25, e202300754. (invited concept review)
Yu-Fei Ao,* Shuxin Pei, Chao Xiang, Marian J. Menke, Lin Shen,* Chenghai Sun, Mark Dörr, Stefan Born, Matthias Höhne, Uwe T. Bornscheuer,* "Structure- and data-driven protein engineering of transaminases for improving activity and stereoselectivity", Angew. Chem. Int. Ed. 2023, 62, e202301660.
Chao Xiang,+ Yu-Fei Ao,+ Matthias Höhne, Uwe T. Bornscheuer,* "Shifting the pH optima of (R)-selective transaminases by protein engineering", Int. J. Mol. Sci. 2022, 23, 15347.
Yu-Fei Ao,* Hui-Juan Hu, Cheng-Xin Zhao, Peng Chen, Tingting Huang, Hui Chen,* Qi-Qiang Wang, De-Xian Wang, Mei-Xiang Wang, "Reversal and amplification of the enantioselectivity of biocatalytic desymmetrization toward meso heterocyclic dicarboxamides enabled by rational engineering of amidase", ACS Catal. 2021, 11, 6900-6907.
Peng-Wei Xu, Xiao-Yuan Cui, Chen Chen, Feng Zhou, Jin-Sheng Yu, Yu-Fei Ao,* Jian Zhou,* "Enantioselective synthesis of Cα‑tetrasubstituted N‑hydroxyl-α-amino nitriles via cyanation of ketonitrones using Me2(CH2Cl)SiCN", Org. Lett. 2021, 23, 8471-8476.
Hui-Juan Hu, Qi-Qiang Wang, De-Xian Wang, Yu-Fei Ao,* "Modification of the enantioselectivity of biocatalytic meso-desymmetrization for synthesis of both enantiomers of cis-1,2-disubstituted cyclohexane by Amidase engineering", Adv. Synth. Catal. 2021, 363, 4538-4543.
Hui-Juan Hu, Qi-Qiang Wang, De-Xian Wang, Yu-Fei Ao,* "Enantioselective biocatalytic desymmetrization for synthesis of enantiopure cis-3,4-disubstituted pyrrolidines", Green Synth. Catal. 2021, 2, 324-327.
Hui-Juan Hu, Peng Chen, Yu-Fei Ao,* Qi-Qiang Wang, De-Xian Wang, Mei-Xiang Wang, "Highly Efficient Biocatalytic Desymmetrization of Meso Carbocyclic 1,3-Dicarboxamides: A Versatile Route for Enantiopure 1,3-Disubstituted Cyclohexanes and Cyclopentanes", Org. Chem. Front. 2019, 6, 808-812.
Yu-Fei Ao,* Li-Bin Zhang, Qi-Qiang Wang, De-Xian Wang, Mei-Xiang Wang, "Biocatalytic desymmetrization of prochiral 3-aryl and 3-arylmethyl glutaramides: Different remote substituent effect on catalytic efficiency and enantioselectivity", Adv. Synth. Catal. 2018, 360, 4594-4603.
敖宇飞,* 王其强，王德先. "有机合成中腈的去对称化生物转化反应研究进展", Chin. J. Org. Chem. 2016, 36, 2333-2343.
Yu-Fei Ao, De-Xian Wang, Liang Zhao, Mei-Xiang Wang*, "Synthesis of quaternary carbon-containing and functionalized enantiopure 3-carbamoylcyclo-pentanecarboxylic acids from biocatalytic enantioselective desymmetrization of meso cyclopentane-1,3-dicarboxamides", Chem. Asian J. 2015, 10, 938-947.
Yu-Fei Ao, De-Xian Wang, Liang Zhao, Mei-Xiang Wang*, "Biotransformations of racemic 2,3-allenenitriles in biphasic systems: Synthesis and transfor-mations of enantioenriched axially chiral 2,3-allenoic acids and their derivatives", J. Org. Chem. 2014, 79, 3103-3110.
Yu-Fei Ao, Dong-Hui Leng, De-Xian Wang, Liang Zhao, Mei-Xiang Wang*, "Efficient synthesis of highly enantiopure β-lactam derivatives from biocatalytic transformations of amides and nitriles", Tetrahedron 2014, 70, 4309-4316.